You use the formula c1 x v1 = c2 x v2 (where 1 means the initial cell suspension and 2 the diluted one): 56000 cells/mL x v1 = 20000 cells/mL x 5mL and you solve for v1: v1 = (20000 cells/mL x 5mL) / (56000 cells/mL) = 1.786mL. But you probably want to have some extra just in case, so let’s say you need to have 100000 cells at the final concentration of 20000 cells/mL (which means you will end up with (100000 cells) / (20000 cells/mL) = 5mL of diluted solution). The total number of cells you will need is 2000 cells x 46 wells = 92000 cells. In each well, you’re going to have 2000 cells in 100uL (0.1mL) so the concentration will be 2000 cells/0.1mL = 20000 cells/mL.Ģ) needs a bit more calculations. So there’s essentially two things you need to calculate: 1) What’s the concentration you need in each well 2) How much you need to dilute the original cell suspension to get to that concentration.ġ) is quite easy.
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